Automation in the clinical microbiology laboratory clinical. In the microbiology lab we use aseptic technique to. Standardisation of the diagnostic process through the application of smis helps to assure the equivalence of investigation strategies in different laboratories across the. A small amount of sample is placed on the side of the agar plate either with a swab, or as a drop from an inoculating loop. If either media is found to be contaminated, the following data should. This is a test commonly used when trying to identify gramnegative enteric bacteria, all of which are glucose fermenters but only some of which produce gas. Prevent contamination of the specific microorganism we are working with. Clinical laboratory microbiology is part of pearsons clinical laboratory science series of textbooks, which is designed to balance theory and practical applications in a way that is engaging and useful to. Microbiology lab safety university of minnesota duluth. Holding shaft by end with colored cap, collect specimen with the swab. Inoculation is a set of methods of artificially inducing immunity against various infectious diseases.
Uk standards for microbiology investigations issued by the. Laboratory methods in microbiology is a laboratory manual based on the experience of the authors over several years in devising and organizing practical classes in microbiology to meet the requirements of. General microbiology laboratory manual by rachel m watson. The grade for general microbiology is based on a total of 800 points 600 from lecture, 200 from lab. Whether youre studying microbiology in high school or as a college student, youll need to write a number of lab reports. Microbiology specimen collection and transport guide. This book is an attempt to collect a wide range of molecular information about all aspects of bacterial pathogens. Persons who work in a microbiology lab may handle infectious agents in additional to. Sterilization of an inoculating tool using a microincinerator video. Thermo scientific microbiological and refrigerated incubators are designed with your samples in mind to produce results you can count on. Pharmacology in the catheterization laboratory wiley. Aseptic technique microbiology resource center truckee. Text book on medical bacteriology for medical laboratory technology students are not available as need, so this lecture note will alleviate the acute shortage of text books and reference materials on medical.
Successful incubation is an essential step in your everyday workflows. The micro lab website, your textbook, the web and assorted books available in lab will be the reference materials necessary for you to successfully complete the next several weeks of lab work. Automation in the clinical microbiology laboratory. Label all tubes and plates and place them on the shelf in the incubator corresponding to your lab section. A carefully arranged laboratory bench basic pract book 2006 21106 11. A sterile loop is then used to spread the bacteria out in one direction from the initial site of inoculation. Laboratory safety adapted from the american society of microbiology website. This indepth examination of the specific types of pharmacological agents used in the cardiac catheterization lab as well as those routinely prescribed for cardiac patients evaluates drugs with respect to their. Using an inoculating loop, remove a loopful of suspension from one of the tubes. Dispose of all materials when the experiment is completed, being sure to remove all markings from the glassware.
Aseptic technique and the transfer of microorganisms. Polio virus from the hogle lab at harvard, url unknown many of the images in the smallpox part of this file come from fenner, henderson, arita et al. Inoculate an agar slant or plate with the frozen stock culture. Field size calculation cell size estimation video unit 3. The inoculation microbiology definition is slightly different from the way that people usually use the term in terms of health, vaccines and immunology. If you have any questions about safe laboratory practices, ask your instructor. Prevent contamination of the room and personnel with the. The microbiology manager or senior technician can record the results for the 7 day and 15 day checks, or 14 full days, into the work book. Start studying microbiology lab practical exam 1 inoculation of media, isolation of bacteria and aseptic techniques.
Nwfhs laboratory procedures manual second edition, june 2004 chapter 5 page 2 i. It was with this in mind that the course behind this book was first conceived and developed. Clinical bacteriology an overview sciencedirect topics. The pharmaceutical microbiology manual pmm evolved from the sterility analytical manual and is a supplement to the united states. The techniques and activities included in this book, and the microorganisms suggested, present minimum risk given good practice. The labreport genre does have several sections youll need to meet in your report, which include. Start studying microbiology lab exercise 4 transfer of bacteria aseptic techniques. Usa home product directory labware microbiology equipment inoculation. Hicks 1 there is a certain element of risk in anything you do, but the potential risks in a microbiology course are greater. Microbiological incubators, also called heatonly or standard incubators have heating elements, and can provide incubation temperatures that are above ambient temperature only. In microbiology, inoculation is defined as introducing microorganisms into a culture where they can grow and reproduce. Microbiological incubators thermo fisher scientific us.
This indepth examination of the specific types of pharmacological agents. However, systems are emerging for the clinical microbiology laboratory with the potential to automate almost all areas of testing, including inoculation of primary culture plates, detection of. Basic practical microbiology why microbiology matters. General microbiology, leaving the particular fields of dairy. Its general structure is, therefore, not unlike that of the classical textbooks. Sep 25, 2019 in microbiology, streaking is a technique used to isolate a pure strain from a single species of microorganism, often bacteria. Laboratory exercises in microbiology cuny academic works.
Inoculation of the media with the prepared culture. You will perform general biochemical tests on an unknown organism. Laboratory methods in microbiology is a laboratory manual based on the experience of the authors over several years in devising and organizing practical classes in microbiology to meet the requirements of students following courses in microbiology at the west of scotland agricultural college. Wash your hands with disinfectant soap when you arrive at the lab and again before you leave. According to apic text of infections control and epidemiology. Aseptic technique, dilution, streaking, and spread. Keeping a complete and accurate record of experimental methods and data is a vital part of science and engineering. Microbiologists use aseptic technique for a variety of procedures such as transferring cultures. Since it comprises most of the contents of course outline on medical bacteriology to nursing, pharmacy and environmental science. Text book on medical bacteriology for medical laboratory technology students are not available as need, so this lecture note will alleviate the acute shortage of text books and reference materials on medical bacteriology. Laboratory manual and workbook in microbiology applications to patient care 7th edition josephine a.
Maheshwari is a good book for the students of microbiology and biotechnology specially in the field microbial culture. Streak the loop across the surface of the agar medium using the either the pattern shown in fig. Pdf on oct 1, 2016, abu nayeem and others published book on microbiology laboratory find, read and cite all the research you need on researchgate. Oct 04, 2019 whether youre studying microbiology in high school or as a college student, youll need to write a number of lab reports. A plastic straw and wood applicator stick serve as a simple, inexpensive, and disposable inoculation unit for fungal studies. Nwfhs laboratory procedures manual second edition, june 2004 chapter 5 page 4 9. Instructions for using your laboratory notebook please read before coming to lab 3. Difco publishes a book cleverly named the difco manual that lists th e application, composition, history of development and other useful information for each medium it supplies. More generally, it can also be defined as introducing a certain substance. Microbiology lab practical exam 1 inoculation of media. Report any problems with your microscope to your instructor.
Microbiology lab exercise 4 transfer of bacteria aseptic. It was with this in mind that the course behind this book. Methods of culturing microorganisms 3 five basic techniques 4 fig. This disease differs from profuse watery diarrhea, as is commonly seen in choleraic diarrhea or in. The labreport genre does have several sections youll need to meet in. In some individuals suffering from shigellosis, however. Inoculation and other aseptic procedures essential points 8 using a wire loop 8 using a pipette 9. For each biochemical test you perform, make sure to record the following in your lab book.
Pharmacology in the catheterization laboratory wiley online. Learn vocabulary, terms, and more with flashcards, games, and other study tools. The purpose of this laboratory manual is to make the. In microbiology, streaking is a technique used to isolate a pure strain from a single species of microorganism, often bacteria. Aseptic an environment or procedure that is free of contamination by pathogens bauman, robert w. Label the tubes or the tube rack with type of medium, preparers initials, and date. Maheshwari is a good book for the students of microbiology and biotechnology specially in the field.
June 2002 overview this microbiology laboratory manual is designed especially for the nonmajors, health science. The primary mission of the clinical bacteriology laboratory is to assist. Remove white cap from top of transport tube and discard. Practical lab reports 18 introduction to microbiology. Chapter 5 bacteriology jason woodland usfws pinetop fish health center pinetop, arizona. Pineapple research station k erala agricultural university, vazhakulam686 670, muvattupuzha. Samples can then be taken from the resulting colonies and a microbiological culture can be grown on a new plate so that the organism can be identified, studied, or tested. Laboratory exercises in microbiology mclaughlin and petersen iii. Routine inoculation of agar slopesdeeps and small volumes of liquid media up. Lift the edge of the lid just enough to insert the loop 4. The terms inoculation, vaccination, and immunization are often used synonymously, but there are some important differences between them. General biochemical tests microbiology resource center. Required lab supplies lab coat there is a limited supply of lab coats in the lab. Inoculate a second tsa plate by the following procedure.
Appendix reduction in the incidence of certain diseases after the introduction of vaccination. Polio virus from the hogle lab at harvard, url unknown. Microbiology module methods of isolation of bacteria microbiology 116 notes streak plates 1. Return all class pure cultures to the instructors lab bench. Gram staining is a procedure used to differentiate between two different types of bacteria grampositive and gramnegative. Morphology and classification of bacteria microbiology module microbiology notes 1. Streakplate and spreadplate inoculations for separation of microorganisms in a. Samples can then be taken from the resulting colonies and a. Microbiology laboratory manual prepared by abdelraouf elmanama. The practice originated in the east before being imported to the western world. The microbiology society, in association with the microbiology in schools advisory committee misac has also produced a book of 21 practical investigations which complements the manual practical microbiology for secondary schools is suitable for use with key stages 3, 4 and post16 and the equivalent scottish qualifications. This disease differs from profuse watery diarrhea, as is commonly seen in choleraic diarrhea or in enterotoxigenic escherichia coli diarrhea, in that the dysenteric stool is scant and contains blood, mucus, and inflammatory cells. This book was written to meet the growing demand for complete, detailed and accurate cardiac pharmacology information. In the microbiology lab, the specimen will be centrifuged to concentrate any cells in a sediment.
Microbiology is a field of science devoted to the study of organisms that are too small to see, and therefore an engaging laboratory experience is often the key to capturing students interest. Aseptic technique is a fundamental and important laboratory skill in the field of microbiology. It tests an organisms ability to ferment the sugar glucose as well as its ability to convert the end product of glycolysis, pyruvic acid into gaseous byproducts. The instructions below are designed to keep you safe in the laboratory. Gramnegative, facultative anaerobes of the genus shigella are the principal agents of bacillary dysentery. This manual has been written for a righthanded person. Microbiology module morphology and classification of bacteria microbiology 2 notes zmost bacteria possess peptidoglycan, a unique polymer that makes its synthesis a good target for. Why is it important to keep a good laboratory notebook.